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17 June 2002 Multicolor two-photon scanning microscopy using a 16-channel photomultiplier
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Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002)
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States
Two-photon scanning microscopy has been successfully applied in studying tissue structures and biochemistry with subcellular spatial resolution. We developed a 16-channel two-photon scanning microscope with high-performance single photon counting readout electronics. The apparatus incorporates a mode-locked Ti:Sapphire laser, a scanning microscope, a spectrograph with multi-anode PMT (16 channels with ~ 7 to 10 nm spectral resolution each), and a 16-channel photon counting card (PhCC) with integrated high-speed data link (payload 1.2 Gbps). Each PhCC detection channel features single-photon sensitivity and 100 MHz photon counting bandwidth. The multi-color single-photon counting detection scheme allows ultra-sensitive measurements in a broad spectrum of biomedical applications. We present spectroscopic studies of ex vivo tissue autofluorescence.
© (2002) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Christof Buehler, Ki Hean Kim, Urs Greuter, Nick Schlumpf, and Peter T. C. So "Multicolor two-photon scanning microscopy using a 16-channel photomultiplier", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002);


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