17 June 2002 Multiwavelength TCSPC lifetime imaging
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Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470679
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States
Abstract
We present a novel time-correlated single photon counting (TCPSC) imaging technique that allows time-resolved multi-wavelength imaging in conjunction with a laser scanning microscope and a pulsed excitation source. The technique is based on a four-dimensional histogramming process that records the photon density over time, the x-y coordinates of the scanning area and the detector channel number. The histogramming process avoids any time gating or wavelength scanning and therefore yields a near-perfect counting efficiency. Applied to resonance energy transfer (RET) measurements, the setup is capable to record time-resolved fluorescence decays for the donor and the acceptor simultaneously.
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Wolfgang Becker, Axel Bergmann, Christoph Biskup, Thomas Zimmer, Nikolaj Kloecker, Klaus Benndorf, "Multiwavelength TCSPC lifetime imaging", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470679; https://doi.org/10.1117/12.470679
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