17 June 2002 Multiwavelength TCSPC lifetime imaging
Author Affiliations +
Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470679
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States
We present a novel time-correlated single photon counting (TCPSC) imaging technique that allows time-resolved multi-wavelength imaging in conjunction with a laser scanning microscope and a pulsed excitation source. The technique is based on a four-dimensional histogramming process that records the photon density over time, the x-y coordinates of the scanning area and the detector channel number. The histogramming process avoids any time gating or wavelength scanning and therefore yields a near-perfect counting efficiency. Applied to resonance energy transfer (RET) measurements, the setup is capable to record time-resolved fluorescence decays for the donor and the acceptor simultaneously.
© (2002) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Wolfgang Becker, Wolfgang Becker, Axel Bergmann, Axel Bergmann, Christoph Biskup, Christoph Biskup, Thomas Zimmer, Thomas Zimmer, Nikolaj Kloecker, Nikolaj Kloecker, Klaus Benndorf, Klaus Benndorf, } "Multiwavelength TCSPC lifetime imaging", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470679; https://doi.org/10.1117/12.470679

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