Recent advances on Coherent Anti-Strokes Raman scattering (CARS) microscopy

Ji-xin Cheng; Eric O. Potma; Sunney Xie

doi:10.1117/12.470697

17 June 2002 Recent advances on Coherent Anti-Strokes Raman scattering (CARS) microscopy

Ji-xin Cheng, Eric O. Potma, Sunney Xie

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Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470697
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Recent advances in CARS microscopy provided exciting possibilities for imaging living cells with high sensitivity, high 3D spatial resolution and chemical specificity based on vibrational spectroscopy. Epi-detection avoids the forward-going water signal and allows imaging of small features inside cells. Polarization-sensitive detection improves the vibrational contrast in CARS microscopy via suppression of the non-resonant background. High-speed CARS imaging of living cells is realized by raster scanning two collinearly overlapped near infrared picosecond laser beams. Vibrational mapping of lipids and proteins inside living cells is achieved. CARS imaging of unstained cells during mitosis and apoptosis is carried out. Incorporation of CARS microscopy with the photon correlation technique allows probing diffusion dynamics of 50-nm beads with chemical selectivity.

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Ji-xin Cheng, Eric O. Potma, and Sunney Xie "Recent advances on Coherent Anti-Strokes Raman scattering (CARS) microscopy", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470697

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KEYWORDS

Microscopy

Cell death

CARS tomography

Microscopes

Raman spectroscopy

Confocal microscopy

Diffusion

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