Two-color correlation spectroscopy

Gregor Jung; Thomas Hellerer; Bjorn Heinz; Andreas Zumbusch

doi:10.1117/12.470674

17 June 2002 Two-color correlation spectroscopy

Gregor Jung, Thomas Hellerer, Bjorn Heinz, Andreas Zumbusch

Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470674
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Fluorescence correlation spectroscopy (FCS) has turned out to be a useful tool for investigations of the population of dark states of the GFP. Using common FCS techniques, it is however not possible to reveal the nature of the dark state. We developed a simultaneous two-color excitation scheme which allows us to collect information about the dark state absorption spectra. Apart from the identification of dark states, two-color FCS opens up ways for the detailed investigation of the photodynamics of molecules with multiple dark states. It will be shown how the increase in fluorescence signal obtained by double resonance excitation can be exploited for obtaining brighter images in live cell and single molecule microscopy. The experimental setup employed for the two-color excitation can easily be extended to generate non-linear vibrational contrast. First examples of this new Coherent Anti-Stokes Raman Scattering (CARS) correlation spectroscopy will be presented.

Citation Download Citation

Gregor Jung, Thomas Hellerer, Bjorn Heinz, and Andreas Zumbusch "Two-color correlation spectroscopy", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470674

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