Two-photon excitation and confocal microscopy investigation of nanostructured polyelectrolyte shells

Alberto Diaspro; Ornella Cavalleri; Daniela Silvano; Silke Krol; Alessandra Gliozzi

doi:10.1117/12.470696

17 June 2002 Two-photon excitation and confocal microscopy investigation of nanostructured polyelectrolyte shells

Alberto Diaspro, Ornella Cavalleri, Daniela Silvano, Silke Krol, Alessandra Gliozzi

Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470696
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Two photon excitation (TPE) and confocal laser scanning microscopy (CLSM) allow obtaining structural and functional information at sub-micron resolution of three-dimensional specimens. We are interested in the relationship between the architectural motifs and functional properties of organized assemblies of nanostructured polyelectrolyte shells. Stepwise adsorption of oppositely charged polyelectrolytes onto micrometer sized organic and inorganic cores followed by core dissolution results in the formation of hollow polyelectrolyte shells, named nanocapsules. Nanocapsules can be used as protected environments for guest molecules allowing controlled release or entrance of substances. They are of both biological and medical interest since can be used for the controlled release and targeting of drugs as well as for the protection of enzymes and proteins. The structure and the permeability properties of the shells in hydrated state were investigated by a TPE architecture based on a compact CLSM, Nikon PCM2000, in which the ability to operate as a standard CLSM was preserved. Imaging was also probed by scanning force microscopy measurements on dried shells. We also plan to check other strategies for core removal and to perform confined experiments into the nanocaspules, including photopatterning and photobleaching ones.

Citation Download Citation

Alberto Diaspro, Ornella Cavalleri, Daniela Silvano, Silke Krol, and Alessandra Gliozzi "Two-photon excitation and confocal microscopy investigation of nanostructured polyelectrolyte shells", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470696

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available