High-resolution 3D reconstruction from serial sections: microscope instrumentation, software design, and its implementations

Fons J. Verbeek; Paul J. Boon

doi:10.1117/12.467833

15 May 2002 High-resolution 3D reconstruction from serial sections: microscope instrumentation, software design, and its implementations

Fons J. Verbeek, Paul J. Boon

Author Affiliations +

Proceedings Volume 4621, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX; (2002) https://doi.org/10.1117/12.467833
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

This paper describes a set-up for high-resolution imaging with a conventional microscope that allows producing a 3D-image set from which a 3D model can be derived. The 3D-image set, is de facto, a gray value voxel model. In order to obtain such 3D image set a good administration needs to be maintained at acquisition and moreover, the acquisition must be realized in two steps. High-resolution images are built of image tiles and sophisticated algorithms are required to build a coherent image from the tiles.

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Fons J. Verbeek and Paul J. Boon "High-resolution 3D reconstruction from serial sections: microscope instrumentation, software design, and its implementations", Proc. SPIE 4621, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX, (15 May 2002); https://doi.org/10.1117/12.467833

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