15 May 2002 High-resolution 3D reconstruction from serial sections: microscope instrumentation, software design, and its implementations
Author Affiliations +
Abstract
This paper describes a set-up for high-resolution imaging with a conventional microscope that allows producing a 3D-image set from which a 3D model can be derived. The 3D-image set, is de facto, a gray value voxel model. In order to obtain such 3D image set a good administration needs to be maintained at acquisition and moreover, the acquisition must be realized in two steps. High-resolution images are built of image tiles and sophisticated algorithms are required to build a coherent image from the tiles.
© (2002) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Fons J. Verbeek, Fons J. Verbeek, Paul J. Boon, Paul J. Boon, } "High-resolution 3D reconstruction from serial sections: microscope instrumentation, software design, and its implementations", Proc. SPIE 4621, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX, (15 May 2002); doi: 10.1117/12.467833; https://doi.org/10.1117/12.467833
PROCEEDINGS
12 PAGES


SHARE
Back to Top