5 June 2002 Changes in quantitative oxygen uptake rate and redox state of cytochromes as a function of normal, critical, and anoxic conditions
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Proceedings Volume 4623, Functional Monitoring and Drug-Tissue Interaction; (2002) https://doi.org/10.1117/12.469463
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States
Abstract
Isolated mitochondria were used to investigate quantitative oxygen uptake rate during oxidative phosphorylation. At the PO2 of 70 mmHg the reaction was initiated. During the initial part of oxidative phosphorylation the turnover rate increased. The recorded PO2 slope decayed linearly over time from 60 to about 10 mmHg, which is correlated to a constant oxygen uptake rate. Below 10 mmHg the PO2 deviated from linearity indicating a decreasing O2 uptake of mitochondria. No change in redox state of respiratory enzymes was observed. At very low PO2 values the scattering of mitochondria decreased and finally at total anoxia a very fast drop of backscattered light intensity and a total reduction of cytochrome was observed.
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Klaus H. Frank, Klaus H. Frank, Valerie Steinbach, Valerie Steinbach, Marc Dammann, Marc Dammann, Manfred D. Kessler, Manfred D. Kessler, } "Changes in quantitative oxygen uptake rate and redox state of cytochromes as a function of normal, critical, and anoxic conditions", Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); doi: 10.1117/12.469463; https://doi.org/10.1117/12.469463
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