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5 June 2002 Imaging of dynamic alteration of functional structures
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Proceedings Volume 4623, Functional Monitoring and Drug-Tissue Interaction; (2002)
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States
Subcellular structures of liver are mainly caused by organelles of hepatocytes and endothelial cells when VIS and NIR are used for irradiation. Total tissue anoxia induces very fast shrinking of mitochondria by switching the oxygenator of the perfusion system from normoxia to total anoxia. This oxygen reaction causes a drastic decrease of backscattered light. For further investigations we used a protocol of three different conditions: 1. Stimulation of subcellular structures in Hb-free perfused liver by application of hypoxia and normoxia, 2. Effects of erythrocytes, 3. Measurements of tissue pigments. Investigations performed by use of our 2D- and 3D-imaging system revealed that the subcellular space with its organelles represents a highly dynamic system.
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Michael Singer, Manfred D. Kessler, and Markus Boehnert "Imaging of dynamic alteration of functional structures", Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002);

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