Yeast and mammalian metabolism continuous monitoring by using pressure recording as an assessment technique for xenobiotic agent effects

Marziale Milani; Monica Ballerini; Lorenzo Ferraro; E. Marelli; Francesca Mazza; Matteo Zabeo

doi:10.1117/12.469775

4 June 2002 Yeast and mammalian metabolism continuous monitoring by using pressure recording as an assessment technique for xenobiotic agent effects

Marziale Milani, Monica Ballerini, Lorenzo Ferraro, E. Marelli, Francesca Mazza, Matteo Zabeo

Author Affiliations +

Proceedings Volume 4625, Clinical Diagnostic Systems: Technologies and Instrumentation; (2002) https://doi.org/10.1117/12.469775
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Our work is devoted to the study of Saccharomyces cerevisiae and human lymphocytes cellular metabolism in order to develop a reference model to assess biological systems responses to chemical or physical agents exposure. CO₂ variations inside test-tubes are measured by differential pressure sensors; pressure values are subsequently converted in voltage. The system allows to test up to 16 samples at the same time. Sampling manages up to 100 acquisitions per second. Values are recorded by a data acquisition card connected to a computer. This procedure leads to a standard curve (pressure variation versus time), typical of the cellular line, that describe cellular metabolism. The longest time lapse used is of 170 h. Different phases appear in this curve: an initial growth up to a maximum, followed by a decrement that leads to a typical depression (pressure value inside the test-tubes is lower than the initial one) after about 35 h from the beginning of yeast cells. The curve is reproducible within an experimental error of 4%. The analysis of many samples and the low cost of the devices allow a good statistical significance of the data. In particular as a test we will compare two sterilizing agents effects: UV radiation and amuchina.

Citation Download Citation

Marziale Milani, Monica Ballerini, Lorenzo Ferraro, E. Marelli, Francesca Mazza, and Matteo Zabeo "Yeast and mammalian metabolism continuous monitoring by using pressure recording as an assessment technique for xenobiotic agent effects", Proc. SPIE 4625, Clinical Diagnostic Systems: Technologies and Instrumentation, (4 June 2002); https://doi.org/10.1117/12.469775

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