Low-cost and high-throughput multichannel capillary electrophoresis (MCCE) system for DNA analysis

Varoujan David Amirkhanian; Ming-Sun Liu

doi:10.1117/12.472087

21 June 2002 Low-cost and high-throughput multichannel capillary electrophoresis (MCCE) system for DNA analysis

Varoujan David Amirkhanian, Ming-Sun Liu

Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002) https://doi.org/10.1117/12.472087
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

In this paper we present the innovative use of an inexpensive Multi-Channel Capillary-based Electrophoresis (MCCE) system in combination with disposable separation cartridge for routine analysis of DNA fragments. The proposed multi-channel system s base don a novel multiplexed fluorescence detection technology, which provides a rapid and unique solution for DNA analysis. Presently the Capillary Electrophoresis (CE) based technology used for DNA analysis, rely on gas discharge UV-visible lamps or lasers as light sources that are bulky, expensive, and difficult to couple one's light output into optical fibers, for miniaturization of the optical detection system. The light sources hinder the development of small sized, high- throughput and cost-effective genomics instrument. Whereas, the proposed instrument with solid-state light sources and non-moving detection micro-optics, and re-usable cartridge containing multiple separation channels, provide a cost- effective solution for a robust CE instrument. Furthermore, the simplified operation of the MCCE instrument will drastically reduce the cost of DNA analysis, and possibly will be the instrument of choice for forensic DNA and molecular diagnostics applications in the near future.

Citation Download Citation

Varoujan David Amirkhanian and Ming-Sun Liu "Low-cost and high-throughput multichannel capillary electrophoresis (MCCE) system for DNA analysis", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); https://doi.org/10.1117/12.472087

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