Massively parallel microfluidics platform for storage and ultra-high-throughput screening

Colin J. H. Brenan; Tom Morrison; Kristine Stone; Tara Heitner; Arrin Katz; Tanya S. Kanigan; Robert Hess; Soek-Jooh Kwon; Heung-Chae Jung; Jae-Gu Pan

doi:10.1117/12.472061

21 June 2002 Massively parallel microfluidics platform for storage and ultra-high-throughput screening

Colin J. H. Brenan, Tom Morrison, Kristine Stone, Tara Heitner, Arrin Katz, Tanya S. Kanigan, Robert Hess, Soek-Jooh Kwon, Heung-Chae Jung, Jae-Gu Pan

Author Affiliations +

Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002) https://doi.org/10.1117/12.472061
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

We have developed a novel microarray technology for performing very large numbers of biochemical, chemical and cell-based nanoliter volume synthesis, storage and screening operations in a massively parallel manner. The Living Chip is an array of precisely machined through-holes retaining nanoliters of fluid by capillary action. Sample loading, washing and recovery are operations that can be performed manually or with simple automation. Mixing between co- registered through-holes is achieved by stacking two or more precision aligned arrays and optical assay read-outs are in parallel with a CCD imaging system. An automated picker system transfers hits into lower density microtiter plates for further analysis. We will present result demonstrating massively parallel implementation of both homogeneous and inhomogeneous fluidic and cell-based assay systems and applications of the chip for drug compound library storage and management.

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Colin J. H. Brenan, Tom Morrison, Kristine Stone, Tara Heitner, Arrin Katz, Tanya S. Kanigan, Robert Hess, Soek-Jooh Kwon, Heung-Chae Jung, and Jae-Gu Pan "Massively parallel microfluidics platform for storage and ultra-high-throughput screening", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); https://doi.org/10.1117/12.472061

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