Micromolded polymer electrokinetic separation systems with variable volume sampling and integrated optical and conductivity detection

Peter R. Fielden; Sara J. Baldock; Nick J. Goddard; Lee Morrison; Jeff E. Prest; Bernard J. Treves Brown; Michele Zgraggen

doi:10.1117/12.472109

21 June 2002 Micromolded polymer electrokinetic separation systems with variable volume sampling and integrated optical and conductivity detection

Peter R. Fielden, Sara J. Baldock, Nick J. Goddard, Lee Morrison, Jeff E. Prest, Bernard J. Treves Brown, Michele Zgraggen

Author Affiliations +

Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002) https://doi.org/10.1117/12.472109
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The micromoulding of polymers has provided an ideal fabrication route for the construction of single-use integrated devices for electrokinetic microseparations. Our designs have incorporated injector geometry that allows variable injection volumes in microchannels controlled by a combination of programmed hydrodynamics and electrophoresis. The utility of the injection scheme is demonstrated for isotachophoresis separations. Further developments have lead to the incorporation of parallel opposed conductivity detection electrodes in two ways. Firstly, by the injection of conducting polymer into a pre-molded channel system and secondly through the molding of the polymer microchannels around electrodes pre-molded in conducting polymer. This has provided a potential rapid manufacturing route for low cost polymer separation devices. As well as integrated conductivity detection electrodes, simple optical elements have been incorporated into injection molded ITP devices to permit detection of the separated bands by optical means. A simple spherical lens was incorporated into the top section of the device which included the sample and buffer reservoirs. The lens was positioned directly above the conductivity detection electrodes, to permit simultaneous electrochemical and optical detection. The lens was used to perform visible absorption spectroscopy using an Ocean Optics spectrography and tungsten-halogen fiber-optic white light source.

Citation Download Citation

Peter R. Fielden, Sara J. Baldock, Nick J. Goddard, Lee Morrison, Jeff E. Prest, Bernard J. Treves Brown, and Michele Zgraggen "Micromolded polymer electrokinetic separation systems with variable volume sampling and integrated optical and conductivity detection", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); https://doi.org/10.1117/12.472109

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