Application of luminescent CdSe-ZnS quantum dot bioconjugates in immuno- and fluorescence-quenching assays

Phan T. Tran; Ellen R. Goldman; George P. Anderson; J. Matthew Mauro; Hedi M. Mattoussi

doi:10.1117/12.459733

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18 March 2002 Application of luminescent CdSe-ZnS quantum dot bioconjugates in immuno- and fluorescence-quenching assays

Phan T. Tran, Ellen R. Goldman, George P. Anderson, J. Matthew Mauro, Hedi M. Mattoussi

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Proceedings Volume 4636, Nanoscience Using Laser-Solid Interactions; (2002) https://doi.org/10.1117/12.459733
Event: High-Power Lasers and Applications, 2002, San Jose, California, United States

Abstract

Colloidal CdSe-ZnS semiconductor quantum dots (QDs) have tunable narrow-band luminescence emission and high resistance to photodegradation, properties that make them promising for use as fluorescent labels in biotechnological applications such as fluoro-immunoassays and in cellular imaging. We have developed efficient methods for conjugating these inorganic fluorophores to antibodies via the use of a molecular adaptor protein, the B2 domain of streptococcal protein G appended with an electrostatic interaction domain, which acts as a bridge between the QD fluorophore and antibodies. In this approach, coupling of the adaptor to the QD is driven by electrostatic self-assembly, while bridging to the antibody is driven by specific interactions. In the present study, we present fluoro-immunoassay studies employing QD-antibody conjugates for the detection of low levels of the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). We will also explore the use of self-assembled QD-protein-receptor complexes in Foerster energy transfer assays, where the QD emission can be altered by a quenching dye receptor in a QD-protein-receptor conjugate.

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Phan T. Tran, Ellen R. Goldman, George P. Anderson, J. Matthew Mauro, and Hedi M. Mattoussi "Application of luminescent CdSe-ZnS quantum dot bioconjugates in immuno- and fluorescence-quenching assays", Proc. SPIE 4636, Nanoscience Using Laser-Solid Interactions, (18 March 2002); https://doi.org/10.1117/12.459733

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