Refractive index of escherichia coli cells

Aleksei E. Balaev; K. N. Dvoretski; Valeri A. Doubrovski

doi:10.1117/12.475627

16 July 2002 Refractive index of escherichia coli cells

Aleksei E. Balaev, K. N. Dvoretski, Valeri A. Doubrovski

Proceedings Volume 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III; (2002) https://doi.org/10.1117/12.475627
Event: Saratov Fall Meeting 2001, 2001, Saratov, Russian Federation

Abstract

Experimental values of the extinction of Escherichia coli cells water suspension by Bryant et al. are used for the refractive index determination of these bacteria. The real suspension of E. coli cells is modeled as a system of identical randomly oriented homogeneous circular cylinders. This model permits one to determine the volume average value of the refractive index of the optically soft particles with a narrow size distribution. The discrete Shifrin method is used to calculate the extinction cross section of the finite cylinders. The results indicate that the relative refractive index is practically independent of the wavelength of the incident radiation in the range from 0.4 micrometers to 0.8 micrometers . The mean value of 1.0387 in this range corresponds to the absolute refractive index value equal to 1.384 at 0.589 micrometers . The obtained value of refractive index of E. coli cells is somewhat lower than the result of immersion refractometry measured by Bryant et al.

Citation Download Citation

Aleksei E. Balaev, K. N. Dvoretski, and Valeri A. Doubrovski "Refractive index of escherichia coli cells", Proc. SPIE 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III, (16 July 2002); https://doi.org/10.1117/12.475627

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