Visualization of intracellular Ca2+ dynamics with third-harmonic generation microscopy

R. Barille; S. Rivet; Lionel Canioni; Laurent Sarger; Pierre Vacher; Pierre Voisin

doi:10.1117/12.468897

30 May 2002 Visualization of intracellular Ca²⁺ dynamics with third-harmonic generation microscopy

R. Barille, S. Rivet, Lionel Canioni, Laurent Sarger, Pierre Vacher, Pierre Voisin

Proceedings Volume 4749, ICONO 2001: Novel Trends in Nonlinear Laser Spectroscopy and Optical Diagnostics and Lasers in Chemistry, Biophysics, and Biomedicine; (2002) https://doi.org/10.1117/12.468897
Event: XVII International Conference on Coherent and Nonlinear Optics (ICONO 2001), 2001, Minsk, Belarus

Abstract

Measurements by Laser scanning Third Harmonic Generation microscopy of Ca²⁺ dynamic release from internal stores and/or calcium influx in biological cells is presented. The Ca²⁺ signal consists of a transient increase in the intracellular concentration. A good correlation is found between these measurements and measurements done with microspectrofluorometry.

Citation Download Citation

R. Barille, S. Rivet, Lionel Canioni, Laurent Sarger, Pierre Vacher, and Pierre Voisin "Visualization of intracellular Ca²⁺ dynamics with third-harmonic generation microscopy", Proc. SPIE 4749, ICONO 2001: Novel Trends in Nonlinear Laser Spectroscopy and Optical Diagnostics and Lasers in Chemistry, Biophysics, and Biomedicine, (30 May 2002); https://doi.org/10.1117/12.468897

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