Cancerigens or radiation action on chromatin determined by spectrofluorimetric methods

Liliana Radu; Doina M. Gazdaru; O. Horer; B. Constantinescu; Ion N. Mihailescu

doi:10.1117/12.530486

19 November 2003 Cancerigens or radiation action on chromatin determined by spectrofluorimetric methods

Liliana Radu, Doina M. Gazdaru, O. Horer, B. Constantinescu, Ion N. Mihailescu

Proceedings Volume 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life; (2003) https://doi.org/10.1117/12.530486
Event: 19th Congress of the International Commission for Optics: Optics for the Quality of Life, 2002, Florence, Italy

Abstract

Steady state and time resolved fluorescence and fluorescence resonance energy transfer (FRET) methods were used in the determination of cancerigens or radiation action on chromatin -- the complex of deoxyribonucleic acid (DNA) with proteins, that exists in eukaryotic cells nuclei. The results indicated modifications in general chromatin structure, DNA strand breaks with the reduction of the double helix DNA proportion (for radiation action) or the growth of the proportion of available DNA for ligand binding (in cancerigens action), proteins structure destruction and an increase of acidic proteins proportion on cancerigens action. The action of other physical or chemical agents may be analyzed by these high performance optical methods.

Citation Download Citation

Liliana Radu, Doina M. Gazdaru, O. Horer, B. Constantinescu, and Ion N. Mihailescu "Cancerigens or radiation action on chromatin determined by spectrofluorimetric methods", Proc. SPIE 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life, (19 November 2003); https://doi.org/10.1117/12.530486

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available