Translator Disclaimer
27 August 2003 A novel flip-chipped integrated single photon detection platform for proteomics
Author Affiliations +
Abstract
Novel integrated sensors will be required for future detection platforms for the measurement of fluorescence and luminescence. The current trend towards integration of optical detectors and the broad advances in optical emitting dyes and proteins will be combined in robust, low-cost, point-of-use, diagnostic equipment. To this end we are experimenting with an integrated optical hybrid sensing device which will combine a flip-chipped, array of solid-state single photon counting detectors with surface mount passive quench circuits on a conventional glass substrate. This flip-chipped arrangement both 1) increases the speed of response of the detector and 2) increases the robustness and ease of integration and reduces single photon detector handling requirements. The potential of integrated solid-state photon detectors will be demonstrated for the real-time quantitative detection of luciferase, a light emitting protein expression reporter molecule. A 15μm solid-state Geiger-mode avalanche photodiode (APD) operating in single photon counting mode will be compared with a standard photomultiplier tube (PMT) for luciferase luminescence detection. Detection levels of 2×106 and 1×107 enzyme molecules will be demonstrated for PMT and Geiger-mode APD respectively. The size of the Geiger-mode APD active area will be shown to be the limiting factor in luciferase signal detection for non-integrated applications. A simple geometric model will show that detection limits of 1×104 are achievable in integrated sensing platforms using room temperature operated single photon counting detectors.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
J. Carlton Jackson, Jonathan West, Aoife M. Moloney, Dan O'Connell, Alan P. Morrison, and Alan Mathewson "A novel flip-chipped integrated single photon detection platform for proteomics", Proc. SPIE 4876, Opto-Ireland 2002: Optics and Photonics Technologies and Applications, (27 August 2003); doi: 10.1117/12.463623; https://doi.org/10.1117/12.463623
PROCEEDINGS
11 PAGES


SHARE
Advertisement
Advertisement
RELATED CONTENT

Optimization of single photon detection model based on GM-APD
Proceedings of SPIE (November 14 2017)
Ultra-compact CMOS single photon detector
Proceedings of SPIE (November 02 2006)
A single photon avalanche detector: SPAD
Proceedings of SPIE (June 11 2007)
Germanium quad cell for single photon detection in the near...
Proceedings of SPIE (September 25 1995)

Back to Top