Studies on cytostatics used as photosensitizing material in photodynamic therapy

Mihail-Lucian Pascu; Leon Danaila; Benone D. Carstocea; Angela Staicu; Sorina Truica; Doina M. Gazdaru

doi:10.1117/12.486634

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15 October 2002 Studies on cytostatics used as photosensitizing material in photodynamic therapy

Mihail-Lucian Pascu, Leon Danaila, Benone D. Carstocea, Angela Staicu, Sorina Truica, Doina M. Gazdaru

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Proceedings Volume 4903, Laser Florence 2001: A Window on the Laser Medicine World; (2002) https://doi.org/10.1117/12.486634
Event: Laser Florence 2001: a Window on the Laser Medicine World, 2001, Florence, Italy

Abstract

Introduction of the photosensitizer properties of cytostatics drus was made, pointing out that the fact that besides the biochemical action of the cytostatics their effects could be enhanced by the exposure to light at different doses. A spectroscopical characterisation of methotrexate and fluorouracil, cytostatic drugs used frequently in cancer therpy was performed. The absorption, emission and excitation spectra were measured for methotrexate solutions in natural saline and sodium hydroxide at concentration in the range 10^-5 -10^-6M and pH 8.4. The absorption, emission and excitation spectra were measured for fluorouracil solutions in natural saline at concentration in the range 10^-4 -10^-5M. The absorption spectrum exhibits spectral bands in the range 250nm -450nm for both drugs. The fluorescence excitatioan for methotrexate was made at 340nm and 370nm, the fluorescence emission was detected in the spectral range 400nm - 500nm with a maximum at 470nm. The fluorescence excitation was measured in teh range 200nm-500nm with the emission centred on 530nm, for Xe lamp irradiation, and 300nm for Hg lamp and laser irradiation. The fluorescence emission spectra was monitored in the spectral range 400nm - 600nm. The effects of irradiation on spectroscopic characteristics of methrotrexate and fluorouracil were investigated. The irraditaion was made using a UV classic lamp with Xe, for the first experimental part and for the second one it was used both a class Hg lamp and a nytorgen pulsed laser.

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Mihail-Lucian Pascu, Leon Danaila, Benone D. Carstocea, Angela Staicu, Sorina Truica, and Doina M. Gazdaru "Studies on cytostatics used as photosensitizing material in photodynamic therapy", Proc. SPIE 4903, Laser Florence 2001: A Window on the Laser Medicine World, (15 October 2002); https://doi.org/10.1117/12.486634

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