12 September 2002 Experimental investigation of the fluorescent spectra of human blood and its components
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Abstract
It has been discussed that the increasing of oxygen content in the blood acts as a key role during the intravascular low level laser irradiation (ILLLI) therapy. In this article, it was investigated why the content of oxygen in the blood would increase after He-Ne laser radiation via measuring the fluorescent spectra of blood and its components. The fluorescent spectra of human blood and its components (plasma, red blood cell) were measured before and after the samples were irradiated by He-Ne laser for 10 minutes, and the changes of spectra were investigated. The fluorescent spectra of human whole blood and components are different. The spectra of whole blood have peaks at 490nm and 614nm; plasma sends strong fluorescence between 410nm and 600nm; the spectra of red blood cell (RBC) have a strong peak at ôI4nm. After irradiated by He-Ne laser for 10 minutes, it was found that there are changes in the spectra of RBC and whole blood. Such a conclusion can be drawn that He-Ne laser inadiation can affect the hemoglobin's energy levels structure and improve the ability of blood carrying oxygen. It is significant to the application of ILLLI.
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Yuqiang Jiang, Yuqiang Jiang, Chang-yong Li, Chang-yong Li, Xiaobo Wang, Xiaobo Wang, Suotang Jia, Suotang Jia, } "Experimental investigation of the fluorescent spectra of human blood and its components", Proc. SPIE 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment, (12 September 2002); doi: 10.1117/12.483005; https://doi.org/10.1117/12.483005
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