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12 September 2002 In-vivo usable chemiluminescence probe carried by nanoparticles
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Chemiluminescence analysis technique is an important method in biology and medicine. Because the reaction between chemiluminescence reagent and high-energy molecule is instantaneous and short-lived, the present chemiluminescence analysis methods have difficulty in living body. In this paper, a novel chemiluminescence probe was designed and used, which was made of human serum albumin and chemiluminescence reagent 2-Methyl-6- (p-methoxyphenyl) -3,7- dihydroimidazo [1,2-a]pyrazin-3-one (MCLA); It can be used to detect the distribution of super oxide anion and singlet oxygen in living body. Electron microscope observation indicated that the diameter of nanoparticles was about 50~150nm and the diameter among 50~80nm can come to 80%. The results in vitro and in vivo showed that the nanoparticles could release MCLA slowly. In vivo experiment, injected the Hematoporphyrin Derivative (HpD) and nanoparticles in the denuded site of mice, stimulated by visible light, then can see distinct lurninesce at the injected site through detecting the two-dimensional image using a highly sensitive Intensified Charged-Coupled Device (ICCD) detector. The results of experiment proved that the time delay resulted from nanoparticles is obvious.
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Min Hao, Da Xing, Juan Wang, and Yonghong He "In-vivo usable chemiluminescence probe carried by nanoparticles", Proc. SPIE 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment, (12 September 2002);

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