12 September 2002 Novel microarray scanner
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Abstract
A novel scanner is developed for detecting the laser induced fluorescence signal emitted from a microarray slide. The scanner uses a line array CCD as detector instead of PMT. In this scanner, a laser line generator is used to generate a laser line to excite the fluorescence. The scanner has high resolution and high sensitivity due to the high sensitive and resolution line array CCD detector and reasonable objective optics system. The microarray slide is driven by a step motor in one direction, which is controlled by PC. Hence a large scanning area can be realized with this scanner. The preliminary experiment results of this novel scanner system are presented in this paper. In this scanner, the sensitivity of the line array CCD is similar to that of PMT, It takes this scanner less than 5 minutes to scan a standard slide (1" x 3"), a different resolution can be achieved, for example, 5 microns, 10 microns, or 14 microns.
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Wenkui Wang, Min Liu, Qiquan Hu, "Novel microarray scanner", Proc. SPIE 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment, (12 September 2002); doi: 10.1117/12.482983; https://doi.org/10.1117/12.482983
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