14 November 2002 Amplification and modelling of bioaffinity detection with terahertz spectroscopy
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Abstract
A new bioaffinity sensor based on pulsed terahertz (THz) spectroscopy is able to sensitively detect the presence of ultra-thin bound biomolecular layers. The protein avidin and lipid biotin are noted for their very high binding affinity, and the ease for which they can be attached to residues with importance in many biosensing applications. We demonstrate the sensitivity of the pulsed THz spectrometer to thin avidin layers and to avidin amplified with micron-sized agarose beads. The experimental results can be simply modelled by considering transmission of the THz radiation at the thin film interfaces. We detect less than 10.3 ng/cm2 avidin, giving the THz system a detection capability of sub-thin solid films better than ellipsometry and reflectometry techniques.
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Samuel P. Mickan, Samuel P. Mickan, Abdellah Menikh, Abdellah Menikh, Jesper Munch, Jesper Munch, Derek Abbott, Derek Abbott, Xi-Cheng Zhang, Xi-Cheng Zhang, } "Amplification and modelling of bioaffinity detection with terahertz spectroscopy", Proc. SPIE 4937, Biomedical Applications of Micro- and Nanoengineering, (14 November 2002); doi: 10.1117/12.488143; https://doi.org/10.1117/12.488143
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