30 January 2003 Detection of microbial cells and preliminary estimation of their physiological state by x-ray microanalysis
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Proceedings Volume 4939, Instruments, Methods, and Missions for Astrobiology VI; (2003) https://doi.org/10.1117/12.486685
Event: Instruments, Methods, and Missions for Astrobiology V, 2002, Moscow, Russian Federation
Abstract
The method of X-ray microanalysis was used for the detection of microbial cells among cell-like particles immediately in the samples of 170 thousand year old ancient Antarctic permafrost sediments. The X-ray spectra and the quantitative parameters of P, S, Ca and K contents in cell-like particles, as well as ratios Ca/K and P/S, were compared with the corresponding data obtained for microbial cells of various physiological states: vegetative cells -- resting forms -- non-viable micromummies. The absence of P and S peaks in X-ray spectra in the most of cell-like particles allowed us to regard them as non-living objects. Among other investigated cell-like particles we were able to find the resting forms of microorganisms with the increased intracellular level of Ca, high Ca/K ratio and low P/S ratio. So, X-ray microanalysis is a promising tool for a primary detection of microbial cells in situ and determination of their physiological state.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Vladimir V. Sorokin, Vladimir V. Sorokin, Andrei L. Mulyukin, Andrei L. Mulyukin, Elena A. Vorobyova, Elena A. Vorobyova, Natalia E. Suzina, Natalia E. Suzina, Vitalii I. Duda, Vitalii I. Duda, Galina I. El-Registan, Galina I. El-Registan, } "Detection of microbial cells and preliminary estimation of their physiological state by x-ray microanalysis", Proc. SPIE 4939, Instruments, Methods, and Missions for Astrobiology VI, (30 January 2003); doi: 10.1117/12.486685; https://doi.org/10.1117/12.486685
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