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14 July 2003 Raman imaging of human macular pigments
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Proceedings Volume 4951, Ophthalmic Technologies XIII; (2003)
Event: Biomedical Optics, 2003, San Jose, CA, United States
We have expanded our efforts to generate high spatial resolution images showing the distibution of carotenoid macular pigments in the human retina using Raman spectroscopy. A low level of macular pigments is associated with an increased risk of developing age-related macular degeneration, a leading cause of irreversible blindness. Using excised human eyecups and resonant excitation of the pigment molecules with narrow bandwidth blue light from a filtered arc lamp, we record Raman images originating from the carbon-carbon double bond stretch vibrations of lutein and zeaxanthin, the carotenoids comprising human macular pigments. Our Raman images reveal significnt differences among subjects, both in regard to absolute levels ss well as spatial distribution within the macula. Since the light levels used to obtain these images are well below established ssafety limits, this technique holds promise for developing a rapid screening diagnostic in large populations at risk for vision loss from age-realted macular degeneration.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Werner Gellermann, Igor V. Ermakov, and Robert W. McClane "Raman imaging of human macular pigments", Proc. SPIE 4951, Ophthalmic Technologies XIII, (14 July 2003); doi: 10.1117/12.477968;

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