Fluorescence-guided resection of intracranial VX2 tumor in a preclinical model using 5-aminolevulinic acid (ALA): preliminary results

Arjen Bogaards; Abhay Varma; Eduardo H. Moriyama; Annie Lin; Anoja Giles; Stuart K. Bisland; Lothar D. Lilge; G. M. Bilbao; Paul J. Muller; Brian C. Wilson

doi:10.1117/12.479426

13 June 2003 Fluorescence-guided resection of intracranial VX2 tumor in a preclinical model using 5-aminolevulinic acid (ALA): preliminary results

Arjen Bogaards, Abhay Varma, Eduardo H. Moriyama, Annie Lin, Anoja Giles, Stuart K. Bisland, Lothar D. Lilge, G. M. Bilbao, Paul J. Muller, Brian C. Wilson

Author Affiliations +

Proceedings Volume 4952, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII; (2003) https://doi.org/10.1117/12.479426
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Fluorescence-guided brain tumor resection may help the neurosurgeon to identify tumor margins that merge imperceptibly into the normal brain tissue and are difficult to identify under white light illumination even using an operating microscope. We compared the amount of residual tumor after white light resection using an operating microscope versus that after fluorescnece-guided resection of an intracranial VX2 tumor in a preclinical model using our previously developed co-axial fluorscence imaging and spectroscopy system, exciting and detecting PpIX fluorescence at 405nm and 635nm respectively. Preliminary results: No fluorescence was present in 3 non-tumor-bearing animals. Fluorescence was present in all 15 tumor-bearing animals after white light resection was completed. To date in 4 rabbits, a decrease in residual tumor was found when using additional fluorescence guided resection compared to white light resection only. Conclusions: ALA induced PpIX fluorescence detects tumor margins not seen under an operation microscope using while light. Using fluorescence imaging to guide tumor resection resulted in a 3-fold decrease in the amount of residual timor. However, these preliminary results indicate that also an additional amount of normal brain is resected, which will be further investigated.

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Arjen Bogaards, Abhay Varma, Eduardo H. Moriyama, Annie Lin, Anoja Giles, Stuart K. Bisland, Lothar D. Lilge, G. M. Bilbao, Paul J. Muller, and Brian C. Wilson "Fluorescence-guided resection of intracranial VX2 tumor in a preclinical model using 5-aminolevulinic acid (ALA): preliminary results", Proc. SPIE 4952, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII, (13 June 2003); https://doi.org/10.1117/12.479426

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