Histomorphometric and proteomic analysis of the acute rabbit corneal tissue response following in vitro exposure to 1540-nm laser light

Thomas E. Eurell; Thomas E. Johnson; William P. Roach

doi:10.1117/12.477905

20 June 2003 Histomorphometric and proteomic analysis of the acute rabbit corneal tissue response following in vitro exposure to 1540-nm laser light

Thomas E. Eurell, Thomas E. Johnson, William P. Roach

Author Affiliations +

Proceedings Volume 4953, Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment III; (2003) https://doi.org/10.1117/12.477905
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

In vitro exposures of explant rabbit corneas to single pulse 1540 nm infrared laser light operating at a pulse width of 0.8 milliseconds resulted in coagulative necrosis of both the corneal epithelium and stroma. Histomorphometric data correlated with increasing tissue radiant exposures. Histologic alterations in the corneal stroma were typical of matrix remodeling within the beam path and reactive to antibodies against matrix metalloproteinase-2. A two-dimensional electrophoretic analysis, using a mini-gel format, was developed to determine if specific corneal protein changes within tissue sections could be detected. Frozen sections taken through the center of the laser lesion were evaluated for proteomic data using tissue isoelectric focusing in the first dimension and polyacrylamide gel electrophoresis in the second dimension. Histomorphometric data describing the extent of the laser lesions were compared to the isoelectric points, molecular weights and relative densities of individual corneal proteins. Increasing radiant exposures of corneal tissues were associated with characteristic histomorphometric and proteomic changes.

Citation Download Citation

Thomas E. Eurell, Thomas E. Johnson, and William P. Roach "Histomorphometric and proteomic analysis of the acute rabbit corneal tissue response following in vitro exposure to 1540-nm laser light", Proc. SPIE 4953, Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment III, (20 June 2003); https://doi.org/10.1117/12.477905

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