29 July 2003 Determination of subsurface tumor localization in animal models with near-infrared (NIR) fluorescence imaging
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We have developed a novel imaging system for determining the localization of tumors labeled by fluorescent contrast agents and embedded several centimeters inside the highly scattered medium. This frequency-domain system utilizes the phased cancellation configuration with a goniometric probe. The instrumentation performance on the phantom test can detect 3 mm diameter sphere filled with 1 nM fluorescent dye, Indocyanine Green (ICG), and 3 cm deep inside the scattering medium with similar optical properties as human breast tissue within a 1 mm localization confidence. Mouse tumor model immersed in appropriate scattering/absorbing medium is used for animal test. Intra-tumor injection of ICG demonstrates the localization of the tumor (5 mm in diameter) submerged 3 cm deep inside the highly scattered medium with 2 mm position error. Results with NIR804-D-Glucosamide on the AR42J tumor bearing nude mouse are also presented with 3 mm localization error. The accuracy of the localization suggest that this system would be helpful to guide the clinical fine-needle biopsy for early breast cancer detection.
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Yu Chen, Yu Chen, Dana M. Blessington, Dana M. Blessington, Zhihong Zhang, Zhihong Zhang, Qian Liu, Qian Liu, Lanlan Zhou, Lanlan Zhou, Chenpeng Mu, Chenpeng Mu, Xavier Intes, Xavier Intes, Samuel I. Achilefu, Samuel I. Achilefu, Hui Li, Hui Li, Min Z. Zhang, Min Z. Zhang, Gang Zheng, Gang Zheng, Britton Chance, Britton Chance, } "Determination of subsurface tumor localization in animal models with near-infrared (NIR) fluorescence imaging", Proc. SPIE 4955, Optical Tomography and Spectroscopy of Tissue V, (29 July 2003); doi: 10.1117/12.478187; https://doi.org/10.1117/12.478187

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