8 July 2003 Imaging of cell layers by differential phase contrast optical coherence microscopy
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Optical coherence tomography (OCT) measures and images the backscattering potential of transparent and semi-transparent samples. Recently, we reported on an extension of OCT with a differential phase contrast (DPC) technique. We have improved the technique and developed a DPC optical coherence microscope (OCM) which allows to image sub-wavelength optical path differences occurring between a narrow beam probing a sample and its surrounding. This allows the visualization of small transversal refractive index variations close to a selected interface. We report on the method and present first images of a single cell layer. The cells act as phase objects, and by imaging the phase properties the contrast is improved as compared to intensity images.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Christoph K. Hitzenberger, Christoph K. Hitzenberger, Markus Sticker, Markus Sticker, Michael Pircher, Michael Pircher, Erich Gotzinger, Erich Gotzinger, Harald Sattmann, Harald Sattmann, Adolf Friedrich Fercher, Adolf Friedrich Fercher, } "Imaging of cell layers by differential phase contrast optical coherence microscopy", Proc. SPIE 4956, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, (8 July 2003); doi: 10.1117/12.477890; https://doi.org/10.1117/12.477890

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