8 July 2003 Imaging of cell layers by differential phase contrast optical coherence microscopy
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Abstract
Optical coherence tomography (OCT) measures and images the backscattering potential of transparent and semi-transparent samples. Recently, we reported on an extension of OCT with a differential phase contrast (DPC) technique. We have improved the technique and developed a DPC optical coherence microscope (OCM) which allows to image sub-wavelength optical path differences occurring between a narrow beam probing a sample and its surrounding. This allows the visualization of small transversal refractive index variations close to a selected interface. We report on the method and present first images of a single cell layer. The cells act as phase objects, and by imaging the phase properties the contrast is improved as compared to intensity images.
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Christoph K. Hitzenberger, Christoph K. Hitzenberger, Markus Sticker, Markus Sticker, Michael Pircher, Michael Pircher, Erich Gotzinger, Erich Gotzinger, Harald Sattmann, Harald Sattmann, Adolf Friedrich Fercher, Adolf Friedrich Fercher, } "Imaging of cell layers by differential phase contrast optical coherence microscopy", Proc. SPIE 4956, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, (8 July 2003); doi: 10.1117/12.477890; https://doi.org/10.1117/12.477890
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