8 July 2003 Imaging of tumor necroses using full-frame optical coherence imaging
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Abstract
Holographic optical coherence imaging (OCI) has been used to acquire depth resolved images in tumor spheroids. OCI is a coherence-domain imaging technique that uses dynamic holography as the coherence gate. The technique is full-frame (en face) and background free, allowing real-time acquisition to a digital camera without motional reconstruction artifacts. We describe the method of operation of the holographic OCI on highly scattering specimens of tumor spheroids. Because of the sub-resolution structure in the sample, the holograms consist primarily of speckle fields. We present two kinds of volumetric data acquisition. One is uses fly-throughs with a stepping reference delay. Another is static holograms at a fixed reference delay with the coherence gate inside the tumor spheroids. At a fixed reference delay, the holograms consist of time-dependent speckle patterns. The method can be used to study cell motility inside tumor spheroids when metabolic or cross-linking poisons are delivered to the specimens.
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Ping Yu, Ping Yu, L. L. Peng, L. L. Peng, M. Mustata, M. Mustata, David D. Nolte, David D. Nolte, J. J. Turek, J. J. Turek, Michael R. Melloch, Michael R. Melloch, Christopher W. Dunsby, Christopher W. Dunsby, Yan Gu, Yan Gu, Paul M. W. French, Paul M. W. French, "Imaging of tumor necroses using full-frame optical coherence imaging", Proc. SPIE 4956, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, (8 July 2003); doi: 10.1117/12.477823; https://doi.org/10.1117/12.477823
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