Biochip using a biofluidic system for detection of E. coli and other pathogens

David L. Stokes; Leonardo R. Allain; Guy D. Griffin; Joel Mobley; Dimitra N. Stratis-Cullum; Alan L. Wintenberg; Robert A. Maples; Tuan Vo-Dinh

doi:10.1117/12.488677

22 July 2003 Biochip using a biofluidic system for detection of E. coli and other pathogens

David L. Stokes, Leonardo R. Allain, Guy D. Griffin, Joel Mobley, Dimitra N. Stratis-Cullum, Alan L. Wintenberg, Robert A. Maples, Tuan Vo-Dinh

Author Affiliations +

Proceedings Volume 4958, Advanced Biomedical and Clinical Diagnostic Systems; (2003) https://doi.org/10.1117/12.488677
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

This work provides an overview of progress made, in our laboratory, towards the development of a practical biochipbased technology with a biofluidics system for the detection of E. coli and other pathogens. Efforts have been devoted towards efficient coupling between a compact biofluidics sample/reagent delivery system and an integrated circuit (IC) biochip, consisting of a 2-dimensional photosensor array, for on-chip monitoring of bioassays. The complementary metal-oxide semiconductor (CMOS) technology has been implemented to design and produce the IC biochip, which features a 4x4 array of independently addressable photodiodes that are integrated with amplifiers, discriminators and logic circuitry on a single platform. The CMOS-based biochip offers the advantages of compactness and low power consumption, making it better suited for field use than other array detectors, including CCDs. The biofluidics system includes a 0.4 mL hybridization chamber, which accommodates disposable sampling platforms embedded with bioreceptors for selective capture of pathogen DNA, proteins, or antibodies in discrete zones. The independently operating photodiodes of the IC biochip offer the capability of monitoring of multiple assays. Highlights of this work include highly sensitive detection of E. coli (<50 organisms) and quantitative capability with a linear dynamic range of 3-5 orders of magnitude for various assays.

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David L. Stokes, Leonardo R. Allain, Guy D. Griffin, Joel Mobley, Dimitra N. Stratis-Cullum, Alan L. Wintenberg, Robert A. Maples, and Tuan Vo-Dinh "Biochip using a biofluidic system for detection of E. coli and other pathogens", Proc. SPIE 4958, Advanced Biomedical and Clinical Diagnostic Systems, (22 July 2003); https://doi.org/10.1117/12.488677

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KEYWORDS

Pathogens

Printing

Organisms

Photodiodes

Control systems

Sensors

Integrated circuits

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