22 July 2003 Clinical applications of slide-based cytometry (SBC)
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Slide-based cytometry (SBC) is a promising new development in clinical diagnostics. The Laser Scanning Cytometer (LSC) was the first such system that became commercially available. Over the years methods have been developed that can be applied in a broad variety of clinical diagnostic settings. The principle of SBC is that fluorochrome labeled specimens are immobilized on microscopic slides which are placed on a conventional epi-fluorescence microscope. Specimens are analyzed by one or two lasers. Data comparable to flow cytometry are generated. But in addition, the position of each individual event is recorded, a feature that allows to re-localize and to visualize each event that has been measured. The major advantage of LSC compared with other cytometric methods is the combination of two features: a) the minimal clinical sample volume needed, and b) the connection of fluorescence data and morphological information about the measured event. We have developed and will present examples of different techniques for application in clinical diagnosis: (1) Immunophenotyping using up to six different fluorochromes at a time, (2) analysis of minimal sample volumes using fine needle aspirate biopsies, and (3) analysis of cells in tissue sections. With these assays and assays developed by others, SBC has proven its wide spectrum of clinical applicability and can be introduced as a standard technology for multiple clinical settings.
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Attila Taernok, Attila Taernok, Andreas O. H. Gerstner, Andreas O. H. Gerstner, "Clinical applications of slide-based cytometry (SBC)", Proc. SPIE 4958, Advanced Biomedical and Clinical Diagnostic Systems, (22 July 2003); doi: 10.1117/12.476122; https://doi.org/10.1117/12.476122


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