Sensing of pH in multiply scattering media with fluorescence lifetime

Eddy Kuwana; Feng Liang; Eva Marie Sevick-Muraca

doi:10.1117/12.476144

22 July 2003 Sensing of pH in multiply scattering media with fluorescence lifetime

Eddy Kuwana, Feng Liang, Eva Marie Sevick-Muraca

Author Affiliations +

Proceedings Volume 4958, Advanced Biomedical and Clinical Diagnostic Systems; (2003) https://doi.org/10.1117/12.476144
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

The fluorescent decay kinetics of an exogenous fluorescent dye injected or immobilized in a polymer implant can be sensitive to the biochemical environment of the tissue and can provide quantitative in vivo biochemical information of the tissue site. In this research project, we develop the rationale for NIR fluorescence spectroscopy of biologically relevant dyes for the sensing of analyte concentration, such as Ca²⁺, pH, glucose, etc. In vivo analytical sensing using fluorescence spectroscopy is complicated not only by the scattering of tissue, but also by the fact that most sensing fluorophores exhibit more than one (multi-exponential) decay lifetime. The objective of this paper is to demonstrate the concept of fluorescence lifetime spectroscopy in scattering media using a pH sensing dye, Carboxy Seminaphthofluorescein-1 (C-SNAFL-1). Work to extrapolate our results to an analyte-sensing dye construct embedded in a polymer are also presented. Supported by the National Institutes of Health (R01 CA67176).

Citation Download Citation

Eddy Kuwana, Feng Liang, and Eva Marie Sevick-Muraca "Sensing of pH in multiply scattering media with fluorescence lifetime", Proc. SPIE 4958, Advanced Biomedical and Clinical Diagnostic Systems, (22 July 2003); https://doi.org/10.1117/12.476144

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