2 July 2003 Analysis of spatial variability in hyperspectral imagery of the uterine cervix in vivo
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The use of fluorescence and reflectance spectroscopy in the analysis of cervical histopathology is a growing field of research. The majority of this research is performed with point-like probes. Typically, clinicians select probe sites visually, collecting a handful of spectral samples. An exception to this methodology is the Hyperspectral Diagnostic Imaging (HSDI) instrument developed by Science and Technology International. This non-invasive device collects contiguous hyperspectral images across the entire cervical portio. The high spatial and spectral resolution of the HSDI instruments make them uniquely well suited for addressing the issues of coupled spatial and spectral variability of tissues in vivo. Analysis of HSDI data indicates that tissue spectra vary from point to point, even within histopathologically homogeneous regions. This spectral variability exhibits both random and patterned components, implying that point monitoring may be susceptible to significant sources of noise and clutter inherent in the tissue. We have analyzed HSDI images from clinical CIN (cervical intraepithelial neoplasia) patients to quantify the spatial variability of fluorescence and reflectance spectra. This analysis shows the spatial structure of images to be fractal in nature, in both intensity and spectrum. These fractal tissue textures will limit the performance of any point-monitoring technology.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Michael James DeWeert, Michael James DeWeert, Jody Oyama, Jody Oyama, Elisabeth McLaughlin, Elisabeth McLaughlin, Ellen Jacobson, Ellen Jacobson, Johan Hakansson, Johan Hakansson, Gary S. Bignami, Gary S. Bignami, Ulf P. Gustafsson, Ulf P. Gustafsson, Paul Troy, Paul Troy, Violeta Poskiene, Violeta Poskiene, Kristina Kriukelyte, Kristina Kriukelyte, Reda Ziobakiene, Reda Ziobakiene, Aurelija Vaitkuviene, Aurelija Vaitkuviene, Sara Palsson, Sara Palsson, Marcelo Soto Thompson, Marcelo Soto Thompson, Unne Stenram, Unne Stenram, Stefan Andersson-Engels, Stefan Andersson-Engels, Sune Svanberg, Sune Svanberg, Katarina Svanberg, Katarina Svanberg, } "Analysis of spatial variability in hyperspectral imagery of the uterine cervix in vivo", Proc. SPIE 4959, Spectral Imaging: Instrumentation, Applications, and Analysis II, (2 July 2003); doi: 10.1117/12.479509; https://doi.org/10.1117/12.479509

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