Optical system to characterize the gross contractile response of a tissue-equivalent collagen matrix

Jami Kern; S. Dan Dimitrijevisch; Tristan Jorge Tayag; Brandon D. Pitt; Tyler H. Summers; Casey G. Davis

doi:10.1117/12.477915

27 August 2003 Optical system to characterize the gross contractile response of a tissue-equivalent collagen matrix

Jami Kern, S. Dan Dimitrijevisch, Tristan Jorge Tayag, Brandon D. Pitt, Tyler H. Summers, Casey G. Davis

Proceedings Volume 4961, Laser-Tissue Interaction XIV; (2003) https://doi.org/10.1117/12.477915
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

We present the theoretical operation and experimental results of a fiber optic interferometer, which we have used to measure the surface displacement of a collagen gel. Dispersed within the collagen gel are fibroblast cells. We describe a measurement system to assist in the detailed study of the biochemical and mechanical processes involved in fibroblast cell contraction. The interferometric system is non-contacting and offers a large dynamic range of measurement. Digital demodulation of a phase generated carrier results in an experimental sensitivity limit on the order of tens of nanometers with a measurement range of up to 2 mm. Computer-controlled data acquisition allows measurement of the contracting gel host over a period of several hours. We describe the optical interferometric system and present experimental results on a contracting collagen gel.

Citation Download Citation

Jami Kern, S. Dan Dimitrijevisch, Tristan Jorge Tayag, Brandon D. Pitt, Tyler H. Summers, and Casey G. Davis "Optical system to characterize the gross contractile response of a tissue-equivalent collagen matrix", Proc. SPIE 4961, Laser-Tissue Interaction XIV, (27 August 2003); https://doi.org/10.1117/12.477915

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