10 July 2003 Fluorescence decay profile measured with spread spectrum excitations
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A novel excitation and detection scheme has been proposed to measure time-resolved fluorescence. A train of continuous excitation pulses modulated by a pseudo-random bit sequence, instead of periodical short pulses of a very low duty factor, is used as the light source to illuminate a fluorescent sample. The cross-correlation between the emission from the sample and the modulation sequence yields the temporal profile of fluorescence decay. We have developed a primitive PC-based time-resolved fluorometer, in which an ultraviolet LED is modulated by a 4095-bit long maximal length pseudo-random bit sequence. The temporal resolution of the fluorometer is up to 1 microsecond. A long lifetime (more than 300 microseconds) europium chelate was used to test our instrument. Its fluorescence decay profile was successfully retrieved, and the measured lifetime is compatible with the specification.
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Nan Guang Chen, Nan Guang Chen, Quing Zhu, Quing Zhu, } "Fluorescence decay profile measured with spread spectrum excitations", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); doi: 10.1117/12.478008; https://doi.org/10.1117/12.478008

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