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10 July 2003 Fluorescence decay profile measured with spread spectrum excitations
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A novel excitation and detection scheme has been proposed to measure time-resolved fluorescence. A train of continuous excitation pulses modulated by a pseudo-random bit sequence, instead of periodical short pulses of a very low duty factor, is used as the light source to illuminate a fluorescent sample. The cross-correlation between the emission from the sample and the modulation sequence yields the temporal profile of fluorescence decay. We have developed a primitive PC-based time-resolved fluorometer, in which an ultraviolet LED is modulated by a 4095-bit long maximal length pseudo-random bit sequence. The temporal resolution of the fluorometer is up to 1 microsecond. A long lifetime (more than 300 microseconds) europium chelate was used to test our instrument. Its fluorescence decay profile was successfully retrieved, and the measured lifetime is compatible with the specification.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Nan Guang Chen and Quing Zhu "Fluorescence decay profile measured with spread spectrum excitations", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); doi: 10.1117/12.478008;

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