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10 July 2003 Raman microscopy of cells: chemical imaging of apoptosis
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Singular value decomposition was applied to the set of the non-resonant Raman spectra, recorded during Raman imaging of the single apoptotic cell. The basis vectors and the corresponding singular values were assessed in terms of their statistical significance. The noise-containing basis vectors were rejected while keeping the meaningful ones. In this way the Raman images of the apoptotic cell were successfully reconstructed from the spectrally-filtered data matrix. The results are demonstrated on the spatial distribution of the DNA, protein and phospholipids, present in the fragments of the apoptotic cell.
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Natallia Uzunbajakava, Jan Greve, and Cees Otto "Raman microscopy of cells: chemical imaging of apoptosis", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003);

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