10 July 2003 Utilization of two-photon FRET to monitor SREBP homodimer and heterodimer formation in living cells
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Abstract
Key players in cholesterol regulation are the members of a family of transcription factors known as the Sterol Regulatory Binding Proteins or SREBPs. The cellular redundancy of these proteins is under investigation, and our findings suggest that where these proteins reside may provide evidence for differences in the molecular dynamics of their transcriptional activity. Specifically, we have found that GFP-tagged SREBP-2 in contrast to SREBP-1 resides in discrete nuclear foci. To further explore functional differences between SREBP-1 and SREBP-2 we have developed an approach to monitor hetero- and homodimer formation by two-photon imaging and spectroscopy of fluorescence resonance energy transfer (TPIS-FRET). TPIS-FRET results will be presented. Collectively, these findings support the possibility that differences in function between SREBP family members may be governed by their localization within the cell.
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Vickie J. LaMorte, Vickie J. LaMorte, Aikaterini Zoumi, Aikaterini Zoumi, Shrimati Datta, Shrimati Datta, Cristen J. Wu, Cristen J. Wu, Timothy Osborne, Timothy Osborne, "Utilization of two-photon FRET to monitor SREBP homodimer and heterodimer formation in living cells", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); doi: 10.1117/12.478034; https://doi.org/10.1117/12.478034
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