9 July 2003 Automatic segementation of histological structures in normal and neoplastic mammary gland tissue sections
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In this paper we present a scheme for real time segmentation of histological structures in microscopic images of normal and neoplastic mammary gland sections. Paraffin embedded or frozen tissue blocks are sliced, and sections are stained with hematoxylin and eosin (H&E). The sections are then imaged using conventional bright field microscopy. The background of the images is corrected by arithmetic manipulation using a "phantom." Then we use the fast marching method with a speed function that depends on the brightness gradient of the image to obtain a preliminary approximation to the boundaries of the structures of interest within a region of interest (ROI) of the entire section manually selected by the user. We use the result of the fast marching method as the initial condition for the level set motion equation. We run this last method for a few steps and obtain the final result of the segmentation. These results can be connected from section to section to build a three-dimensional reconstruction of the entire tissue block that we are studying.
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Rodrigo Fernandez-Gonzalez, Rodrigo Fernandez-Gonzalez, Thomas Deschamps, Thomas Deschamps, Adam Idica, Adam Idica, Ravikanth Malladi, Ravikanth Malladi, Carlos Ortiz de Solorzano, Carlos Ortiz de Solorzano, "Automatic segementation of histological structures in normal and neoplastic mammary gland tissue sections", Proc. SPIE 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X, (9 July 2003); doi: 10.1117/12.477996; https://doi.org/10.1117/12.477996

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