9 July 2003 The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes
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Abstract
Images of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.
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Lisong Yang, Lisong Yang, Cian M. Taylor, Cian M. Taylor, Yury Rakovich, Yury Rakovich, Eithne M. McCabe, Eithne M. McCabe, "The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes", Proc. SPIE 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X, (9 July 2003); doi: 10.1117/12.477978; https://doi.org/10.1117/12.477978
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