The aim of this study was to measure the velocity and velocity profile of 0.3% Intralipid mixture in a 1.5-mm thick glass capillary using Doppler Optical Coherence Tomography (DOCT). First, while still empty, the dimensions of the capillary were measured. The outer diameter was 1.50 mm ± 0.01 mm while the lumen diameter was 1.01 ± 0.01 mm. Then, having filled the capillary with 0.3% solution, the lumen diameter was measured again. The mean refractive index of the solution was calculated and turned out to be 1.36 ± 0.01 mm. During the next stage, flow measurements were performed at an angle of 88° between the illuminating beam and the velocity vector of the fluid. The velocity profile, based on a set of 10 measurements, was calculated from the DOCT signal using a discrete Fourier transform in 32 sections of the capillary. The maximum velocity, located in the middle part of the capillary, was 0.256 ± 0.035 m/s. The results show that the flow velocity profile of 0.3% Intralipid solution can be obtained from a glass capillary.