23 July 2003 In situ identification of bacteria size by light scattering
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Abstract
Optical transmission and light scattering measurements were performed on three species of bacteria with different sizes and shapes (Pseudomonas aeruginosa. Staphylococcus aureus and Bacillus subtilis). The average bacteria size was determined from transmission measurements by using the Gaussian Ray Approximation of Anomalous Diffraction Theory. A rescaled spectra combining multiple angular data was analyzed in the framework of the Rayleigh-Gans theory of light scattering in order to determine particle shape and size distribution. Particle size and shape as determined by both methods are in good agreement with size and shape measured by scanning electron microscopy. These results demonstrate that light scattering may be able to detect and identify microbial contamination in the environment.
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Alvin Katz, Alvin Katz, Alexandra Alimova, Alexandra Alimova, Min Xu, Min Xu, Elizabeth Rudolph, Elizabeth Rudolph, Howard E. Savage, Howard E. Savage, Mahendra Shah, Mahendra Shah, Steven A. McCormick, Steven A. McCormick, Richard B. Rosen, Richard B. Rosen, Robert R. Alfano, Robert R. Alfano, } "In situ identification of bacteria size by light scattering", Proc. SPIE 4965, Optical Diagnostics and Sensing in Biomedicine III, (23 July 2003); doi: 10.1117/12.479188; https://doi.org/10.1117/12.479188
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