18 July 2003 An experimental setup for combining optical tweezers and laser scalpels with advanced imaging techniques
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Abstract
In this paper we will describe a system designed to combine optical tweezers and laser scalpels with confocal as well as epi-fluorescence microscopy. A continuos wave Nd:YVO4 laser is used to produce a dual optical tweezers, where each trap can be individually controlled. A second optical tweezers setup is based on a tunable titanium sapphire laser, which allows us to adjust the wavelength to minimize the damage to the cell under investigation. A pulsed nitrogen laser working at 337 nm forms a laser scalpel. The tweezers and scalpels are both incorporated in an inverted microscope equipped with epi-fluorescence and confocal imaging capabilities. In order to further control the sample we have developed a technique to tailor make the environment closest to the studied objects. Micrometer-sized structures such as channels and reservoirs have been produced in rubber silicon using lithographic methods. In combination with a micro-manipulator, our system can be used to extract single cells from a population of billions for further studies or growth.
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Mattias Goksor, Mattias Goksor, Jonas Enger, Jonas Enger, Kerstin Ramser, Kerstin Ramser, Dag Hanstorp, Dag Hanstorp, } "An experimental setup for combining optical tweezers and laser scalpels with advanced imaging techniques", Proc. SPIE 4966, Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis, (18 July 2003); doi: 10.1117/12.501010; https://doi.org/10.1117/12.501010
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