Immobilization of multiple proteins in polymer microstructures fabricated via laser ablation

Elena P. Ivanova; Andrea Viezzoli; Yulia V. Alekseeva; Gregory M. Demyashev; Dan Valeriu Nicolau Jr.; Luisa Filipponi; Duy K. Pham; Dan V. Nicolau

doi:10.1117/12.483472

18 July 2003 Immobilization of multiple proteins in polymer microstructures fabricated via laser ablation

Elena P. Ivanova, Andrea Viezzoli, Yulia V. Alekseeva, Gregory M. Demyashev, Dan Valeriu Nicolau Jr., Luisa Filipponi, Duy K. Pham, Dan V. Nicolau

Author Affiliations +

Proceedings Volume 4966, Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis; (2003) https://doi.org/10.1117/12.483472
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Investigation of protein-polymeric surface interaction requires reliable practical techniques for evaluation of the efficiency of protein immobilization. In this study the efficiency of protein immobilization was evaluated using three different techniques: (1) protein-binding assay with fluorescent detection and (2) quantification, and (3) atomic force microscopy. This approach enables us to rapidly analyse the adsorption properties of different proteins. The comparative physico-chemical adsorption of α-chymotrypsin, human serum albumin, human immunoglobulin, lysozyme, and myoglobin in the micro-wells fabricated via a localized laser ablation of a protein-blocked thin gold layer (50 nm) deposited on a Poly(methyl ethacrylate) film has been studied. Correlations were observed between the quantitative and qualitative differences depending on both protein and polymeric surface hydrophobicity.

Citation Download Citation

Elena P. Ivanova, Andrea Viezzoli, Yulia V. Alekseeva, Gregory M. Demyashev, Dan Valeriu Nicolau Jr., Luisa Filipponi, Duy K. Pham, and Dan V. Nicolau "Immobilization of multiple proteins in polymer microstructures fabricated via laser ablation", Proc. SPIE 4966, Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis, (18 July 2003); https://doi.org/10.1117/12.483472

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available