Some potential uses of photothermal detection for DNA microarray quantification

Stephane Getin; Patrick Chaton; Francoise Vinet; Ludovic Poupinet; Catherine Pelle; Pierre Puget; Frederic Ginot; Armelle Novelli

doi:10.1117/12.478353

18 July 2003 Some potential uses of photothermal detection for DNA microarray quantification

Stephane Getin, Patrick Chaton, Francoise Vinet, Ludovic Poupinet, Catherine Pelle, Pierre Puget, Frederic Ginot, Armelle Novelli

Author Affiliations +

Proceedings Volume 4966, Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis; (2003) https://doi.org/10.1117/12.478353
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Photothermal deflection technique is a very sensitive mean to measure optical absorbance. This study is aimed at evaluating its potentiality in order to detect hybridation and to monitor quality control. The principle of the technique relies on the fact that nucleic acids present the property to absorb light between 220 nm to 280 nm. A first theoretical approach based on solving light and thermal equations demonstrates the ability of photothermal deflection to detect hybridisation. This point is checked through several experimentations with oligonucleotid with 32 mers targets lengths. An important point to stres is the specificity of the signals obtained and the ability of automation of the reading with image processing algorithms. At last we focus our attention on the ability of this technique to in-situ synthesis process. Our experimental study shows the ability of this characterisation with a detection sensitivity of one base.

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Stephane Getin, Patrick Chaton, Francoise Vinet, Ludovic Poupinet, Catherine Pelle, Pierre Puget, Frederic Ginot, and Armelle Novelli "Some potential uses of photothermal detection for DNA microarray quantification", Proc. SPIE 4966, Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis, (18 July 2003); https://doi.org/10.1117/12.478353

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KEYWORDS

Absorption

Refractive index

Absorbance

Argon ion lasers

Camera shutters

Image processing

Laser beam diagnostics

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