Aggregation strongly influences the pH-profile of the fluorescence of yellow fluorescent protein zFP538

Nadya N. Zubova; Natalia V. Rudenko; Alexander P. Savitsky

doi:10.1117/12.485818

12 September 2003 Aggregation strongly influence the pH-profile of the yellow fluorescent protein zFP538

Nadya N. Zubova, Natalia V. Rudenko, Alexander P. Savitsky

Proceedings Volume 4967, Genetically Engineered and Optical Probes for Biomedical Applications; (2003) https://doi.org/10.1117/12.485818
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Fluorescence of the yellow fluorescent protein zFP538 strongly depends on concentration and starting pH form which pH profile is recorded. pH transitions typical for chromopeptides isolated from zFP538 can be observed for whole protein in diluted solutions. The quenching fluorescence of zFP538 is irreversible upon acidification or alkalization of the low concentrated soultions. In concentrated solutions, according to the data of dynamic light scattering, the protein zFP538 is strongly aggretaed (or oligomerized) and become more stable against acid denaturation. Spectral changes on pH are almost reversible both for fluorescence and absorbance. Two major chromopeptides obtained from zFP538 have different spectral properties and no similarity to the spectral properties of the chromopeptide obtained from GFP.

Citation Download Citation

Nadya N. Zubova, Natalia V. Rudenko, and Alexander P. Savitsky "Aggregation strongly influence the pH-profile of the yellow fluorescent protein zFP538", Proc. SPIE 4967, Genetically Engineered and Optical Probes for Biomedical Applications, (12 September 2003); https://doi.org/10.1117/12.485818

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