12 September 2003 In vivo cytochrome P450 drug metabolizing enzyme characterization using surface-enhanced Raman spectroscopy
Author Affiliations +
Abstract
The development of a rapid, inexpensive, and accurate in vivo phenotyping methodology for characterizing drug-metabolizing phenotypes with reference to the cytochrome P450 (CYP450) enzymes would be very beneficial. In terms of application, in the wake of the human genome project, considerable interest is focused on the development of new drugs whose uses will be tailored to specific genetic polymorphisms, and on the individualization of dosing regimens that are also tailored to meet individual patient needs depending upon genotype. In this investigation, chemical probes for CYP450 enzymes were characterized and identified with Raman spectroscopy. Furthermore, gold-based metal colloid clusters were utilized to generate surface enhanced Raman spectra for each of the chemical probes. Results will be presented demonstrating the ability of SERS to identify minute quantities of these probes on the order needed for in vivo application.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yanfang Li, Kenneth A. Bachmann, Brent D. Cameron, "In vivo cytochrome P450 drug metabolizing enzyme characterization using surface-enhanced Raman spectroscopy", Proc. SPIE 4967, Genetically Engineered and Optical Probes for Biomedical Applications, (12 September 2003); doi: 10.1117/12.478394; https://doi.org/10.1117/12.478394
PROCEEDINGS
6 PAGES


SHARE
Back to Top