Interaction method of photobleaching produced by the interaction between excitation photons and fluorophore molecules in excited states

Tongsheng Chen; Shaoqun Zeng; Qingming Luo

doi:10.1117/12.478400

12 September 2003 Interaction method of photobleaching produced by the interaction between excitation photons and fluorophore molecules in excited states

Tongsheng Chen, Shaoqun Zeng, Qingming Luo

Author Affiliations +

Proceedings Volume 4967, Genetically Engineered and Optical Probes for Biomedical Applications; (2003) https://doi.org/10.1117/12.478400
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

We proposed and analyzed the D-P (Dye-to-Photon) photobleaching mechanism, the interaction between the excitation photon(s) and the fluorophore dye molecules in excited states. At single molecule level, the D-P photobleaching probability with One- and Two-photon excitation (OPE and TPE) should always depend linearly on the probability of fluorophore molecule in excited states. However, at biological imaging level, the dependence of the D-P photobleaching probability on the probability of fluorophore molecules in excited states may depend on the excitation way: the photobleaching states, while the photobleaching rate depends on the high-order power of the probability of fluorophore molecule in the excited states, especially in the case of high excitation intensity.

Citation Download Citation

Tongsheng Chen, Shaoqun Zeng, and Qingming Luo "Interaction method of photobleaching produced by the interaction between excitation photons and fluorophore molecules in excited states", Proc. SPIE 4967, Genetically Engineered and Optical Probes for Biomedical Applications, (12 September 2003); https://doi.org/10.1117/12.478400

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