Digital in-line holography with numerical reconstruction: 4D tracking of microstructures and organisms

Hans Jurgen Kreuzer; M. H. Jericho; I. A. Meinertzhagen; W. Xu

doi:10.1117/12.476875

4 June 2003 Digital in-line holography with numerical reconstruction: 4D tracking of microstructures and organisms

Hans Jurgen Kreuzer, M. H. Jericho, I. A. Meinertzhagen, W. Xu

Proceedings Volume 5005, Practical Holography XVII and Holographic Materials IX; (2003) https://doi.org/10.1117/12.476875
Event: Electronic Imaging 2003, 2003, Santa Clara, CA, United States

Abstract

Using holographic microscopy we have been able to visualize submicron-sized. bacteria in-vivo. A simple holographic method enables us to capture as a single data set the trajectories of micron size objects suspended in water. By subtracting consecutive holograms of a particle suspension and then adding these difference holograms, a final data set is constructed that contains the time evolution of the particle trajectories free from spurious background interference effects. Temporal and spatial resolution at the sub-second and sub-micron levels can easily be achieved. The method is illustrated by recording the motion in 3-D of 5μm diameter latex spheres subject to gravity and electrostatic fields to visualize their micro-fluidic flow. Another example is the 3-D motion of a collection of algae, protozoa and bacteria in water.

Citation Download Citation

Hans Jurgen Kreuzer, M. H. Jericho, I. A. Meinertzhagen, and W. Xu "Digital in-line holography with numerical reconstruction: 4D tracking of microstructures and organisms", Proc. SPIE 5005, Practical Holography XVII and Holographic Materials IX, (4 June 2003); https://doi.org/10.1117/12.476875

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